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Analytical Method Development and Validation Description for Quantitative Assessment of Nitrothal-Isopropyl by HPLC method
Author Name : B.Supraja, N. Venkatasubba Naidu
ABSTRACT
A consistent, systematic, exact and precise high performance liquid chromatographic method for the analysis of pesticide Nitrothal-Isopropyl in its formulations was developed and validated in the present study. The mobile phase consists of a mixture of methanol and water in the proportion 60:40 respectively (v/v). This was found to give sharp peak of Nitrothal-Isopropyl at a run time of 15 min. HPLC analysis of Nitrothal-Isopropyl was carried out at a wave length of 260 nm with a flow rate of 1.2mL/min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.999 in the concentration range of 25% to 125%. The linear regression equation was y= 1866x+760.7. The developed method was employed with a high degree of precision and accuracy for the analysis of Nitrothal-Isopropyl. The method was validated for accuracy, precision, robustness, ruggedness and specificity. The precision, accuracy, sensitivity, short retention time and composition of the mobile phase stipulated that this method is applicable to the evaluation of Nitrothal-Isopropyl.
Keywords: Nitrothal-Isopropyl, HPLC Method, Development and analysis