GC-MS Analysis of Hexane Fractions of Ethanol...

GC-MS Analysis of Hexane Fractions of Ethanol Extract Fruit of Santalum album

Author Name : Ravin Chouhan, Dr. Prakash Solanki

ABSTRACT

The yellow-colored oily fraction of ethanol extract from red black fruits of the Santalum album plant, lead to the identification of the number of long-chain hydrocarbons, alcohols, acids and amino acid compounds. The ethanol eluent yielded a waxy liquid extract of Santalum album fruit, which was rechromatographed on a silica gel column. After the fractionation of this extract by column chromatography, hexane, benzene and chloroform fractions were obtained in lesser quantities. For the present study, we have chosen the hexane fraction (coded as RC-03respectively) which were showed very less separation (tailing or steak) on the TLC plate, therefore they were separated and analyzed by gas chromatography-mass spectrometry (GC-MS) technique from CIL/SAIF Punjab University, Chandigarh. Fraction revealed the presence of a total number of 4 compounds. These compounds were identified by comparing their retention time and covate indexes with that of literature and by interpretation of mass spectra. Some of them were not reported previously from this medicinal plant. Hence Santalum album fruit is a good source of ingredients. The present study aimed to analyze the hexane fraction of ethanol extract from the fruit rinds of Santalum album for the identification of various components of medicinal and other applications.

Keywords: Santalum album, fruits, ethanolextract, hexane fraction, and GC-MS.